FIGURE 2.

ING2 induces the expression of the TGF-β-responsive PAI-1 gene. RNA extracted from untreated or 1 h TGF-β-treated control or ING2-overexpressing Mv1Lu cells was reverse transcribed using oligo(dT) followed by PCR amplification of fragments of PAI-1, ING2, and GAPDH gene products as described under “Reverse Transcription-PCR” under “Experimental Procedures.” The PAI-1, ING2, and GAPDH amplified PCR fragments were resolved, imaged, and quantified (see “Reverse Transcription-PCR” for details). The PAI-1 value for each sample was normalized to its respective GAPDH control and expressed relative to the untreated control. A, a scan of an ethidium bromide-stained agarose gel showing the PAI-1, ING2, and GAPDH reverse transcription-PCR-amplified fragment from a representative experiments that was repeated three times. B, bar graph of the mean (±S.E.) of the PAI-mRNA values from three independent experiments. The asterisk indicates a statistically significant increase from the TGF-β-treated control as determined by Student's t test (p = 0.06).