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The oxygenase domain of mammalian NOS is functional in E. coli. Time-dependent nitrite (left) and the sum of nitrite and nitrate (right) formation in E. coli cultures expressing the following proteins: bsNOS, NOS from B. subtilis; nNOSoxy, oxygenase domain of rat nNOS; nfl, full-length rat nNOS. BL21 stands for control cells that did not express any protein. Data points are the mean of triplicate determinations and are representative of two independent experiments.
