FIGURE 1.

Ethanol induces an increase in TH immunoreactivity in a time- and dose-dependent manner. A, SH-SY5Y cells were treated without (lane 1, control) or with 100 mm ethanol (lanes 2–4) for the indicated times. TH protein levels were analyzed by Western blot with anti-TH antibody, and actin protein levels were detected with anti-actin antibody as an internal control. The histogram depicts the mean percentage change in the ratio of TH to actin ± S.D. from seven experiments. B, cells were treated for 24 h without (lane 1, control) or with different concentrations of ethanol (lanes 2–4). The histogram depicts the mean percentage change in the ratio of TH to actin ± S.D. from three experiments. C, cells were treated without (lane 1, control) or with 100 mm ethanol for 24 h (lane 2), or with 100 mm ethanol for 24 h followed by washing with medium and incubation without ethanol for an additional 24 h (lane 3). The histogram depicts the mean percentage change in the ratio of TH to actin ± S.D. from four experiments. *, p < 0.05; **, p < 0.01, compared with lane 1 (control).