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. 2008 May 9;283(19):12811–12818. doi: 10.1074/jbc.M706216200

FIGURE 4.

FIGURE 4.

Geldanamycin, an inhibitor of heat shock protein 90, inhibits chronic ethanol-induced TH accumulation. A, cells were treated without (lane 1) or with 100 mm ethanol for 24 h (lane 2), or with 100 mm ethanol for 24 h to which different doses of GA were added for the last 9 h of ethanol treatment (lanes 3–5). TH protein levels were analyzed as described above. The histogram depicts the mean percentage change in the ratio of TH to actin ± S.D. from three experiments. *, p < 0.05, lanes 2 versus 1, or 4 versus 2; **, p < 0.01, lanes 5 versus 2. B, cells were treated without (Con), or with 100 mm ethanol for 12 h (Et 12h) or 24 h (Et 24h), or 100 mm ethanol for 24 h in which 1 μm GA was added for the last 9 h (Et 24h/GA 9h). Protein levels of HSP90 and actin were measured by Western blot analysis. The histogram depicts the mean ratio of HSP90 to actin ± S.D. from three experiments.