FIGURE 7.

GDNF decreases ethanol-mediated stabilization and association of TH with HSP90. A, cells were treated with 100 mm ethanol for 24 h alone (Et/-GDNF) or together with 25 ng/ml GDNF added for the last 12 h (Et/+GDNF) before a pulse-chase procedure as described above with the chase times as indicated. The histogram depicts the mean percentage change in radioactive signals of ³⁵S-labeled TH protein from three experiments. *, p < 0.05. B, cells were treated without (Con) or with 100 mm ethanol for 24 h alone (Et) or in combination with 25 ng/ml GDNF added for the last 12 h (Et/GDNF). Co-immunoprecipitation of HSP90 with TH was analyzed as described above. The image is representative of three experiments.