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. 2008 May 9;283(19):12870–12876. doi: 10.1074/jbc.M708949200

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FIGURE 3. — Alternative processing has slowed kinetics. Pulse-chase experiment demonstrating altered kinetics of pro-PM2 (pro) autoprocessing in the presence of E-64d. The parasites were metabolically labeled for 5 min and then chased in complete medium without (control) or with (+E-64d)25 μm E-64d for indicated times followed by immunoprecipitation with anti-PM II antibody and visualization by autoradiography. t_½ values were calculated as 15.0 and 9.5 min for control and E-64d, respectively. E-64d was added to both pulse and chase media.