TABLE 2.

Oligonucleotide sequences used in site-directed mutagenesis of Lpg1905

The codon encoding the relevant amino acid is underlined with base changes in bold.

Sequences	Use
5′-CGCAACAGCTGGTATGGCACTACTACCCCAATCAC-3′	Sense and anti-sense oligonucleotides to mutate Lpg1905 Arg122 to Ala
5′-GTGATTGGGGTAGTAGTGCCATACCAGCTGTTGCG-3′
5′-GACTGGCTTGCTGTAGCTTATAAACTTGATAC-3′	Sense and anti-sense oligonucleotides to mutate Lpg1905 Asn168 to Ala
5′-GTATCAAGTTTATAAGCTACAGCAAGCCAGTC-3′
5′-GGGCGGCGCTTCCGTTGCAATTGTCTTTCCAATG-3′	Sense and anti-sense oligonucleotides to mutate Lpg1905 Gln193 to Ala
5′-CATTGGAAAGACAATTGCAACGGAAGCGCCGCCC-3′