FIGURE 6.

Inhibition of Hpx-dimer by soluble Hpx abrogates TM dimer formation. A, COS7 cells were transfected with the expression plasmids of MT1F with or without either MT1F-ΔCatL1 or MT1F-ΔCatL1ΔTM as indicated and proMMP-2 activation was analyzed as in Fig. 1B. FLAG, anti-FLAG M2 antibody; P, proMMP-2; A, active MMP-2. B, COS7 cells were transfected with expression plasmids for MT1F/NGFRCP with or without MT1F-ΔCatL1 or MT1F-ΔCatL1ΔTM as indicated. Cells were lysed and subjected to Western blotting analyses using anti-phospho-tyrosine antibody (PY, upper panel) and anti-FLAG M2 antibody (lower panel). The relative intensity of the bands detected by PY-20 was analyzed with NIH Image, normalized by the intensity of FLAG bands.