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. 2008 May 9;283(19):13459–13470. doi: 10.1074/jbc.M708506200

TABLE 1.

Fitted ITC data for PR and PRD25N binding to ligands in sodium acetate buffer at pH 5

Protease construct Ligand Binding constant (Ka) ΔH ΔS Stoichiometry
M−1 cal/mol cal/mol [ligand]/[protein]
PR RPB (1.86 ± 0.76) × 107 −3,661 ± 114 21.1 0.95 ± 0.02
DRVa 2.2 × 1011 −12,100 10.5
PRD25N RPB (3.30 ± 0.56) × 106 −4,738 ± 87 14.1 1.00 ± 0.01
DRV (3.17 ± 0.29) × 105 −5,812 ± 206 5.9 1.03 ± 0.03
a

For wild-type PR in 10 mm sodium acetate buffer, pH 5, at 20 °C (data from Ref. 38).