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. 2008 May 9;283(19):13341–13356. doi: 10.1074/jbc.M800153200

FIGURE 5.

FIGURE 5.

T4 and E. coli SSB proteins bind single-stranded 5′-CAG60, 5′-CTG60, and 5′-GTT53 or random DNA with comparable affinity. Gel mobility shift assay of 5′-CTG60 (A) or 5′-CAG60 (B) ssDNA templates bound to either T4 SSB proteins (left panels) or E. coli SSB proteins (right panels). The protein concentration in nanomolar is indicated and given in monomeric units; species are separated on a 10% acrylamide gel. C, gel mobility shift assay for T4 SSB protein binding to 5′-CTG60, 5′-CAG60, 5′-GTT53, and random sequence DNA. Species are resolved on a 3% acrylamide gel. T4 SSB protein concentrations indicated by black triangles are 0, 55 nm, 550 nm, 5.5 mm, and 55 mm.