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. 1982 Jun;43(6):1373–1379. doi: 10.1128/aem.43.6.1373-1379.1982

Kinetic Analysis of Competition Between Sulfate Reducers and Methanogens for Hydrogen in Sediments †

Derek R Lovley 1,2, Daryl F Dwyer 1,2, Michael J Klug 1,2
PMCID: PMC244242  PMID: 16346033

Abstract

The competition between sulfate-reducing and methanogenic bacteria for hydrogen was investigated in eutrophic lake sediments that contained low in situ sulfate concentrations and in sulfate-amended sediments. Sulfate reduction and methane production coexisted in situ in lake surface sediments (0 to 2 cm), but methane production was the dominant terminal process. Addition of 10 to 20 mM sulfate to sediments resulted in a decrease in the hydrogen partial pressure and a concomitant inhibition of methane production over time. Molybdate inhibition of sulfate reduction in sulfate-amended sediments was followed by an increase in the hydrogen partial pressure and the methane production rate to values comparable to those in sediments not amended with sulfate. The sulfate reducer population had a half-saturation constant for hydrogen uptake of 141 pascals versus 597 pascals for the methanogen population. Thus, when sulfate was not limiting, the lower half-saturation constant of sulfate reducers enabled them to inhibit methane production by lowering the hydrogen partial pressure below levels that methanogens could effectively utilize. However, methanogens coexisted with sulfate reducers in the presence of sulfate, and the outcome of competition at any time was a function of the rate of hydrogen production, the relative population sizes, and sulfate availability.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Abram J. W., Nedwell D. B. Hydrogen as a substrate for methanogenesis and sulphate reduction in anaerobic saltmarsh sediment. Arch Microbiol. 1978 Apr 27;117(1):93–97. doi: 10.1007/BF00689357. [DOI] [PubMed] [Google Scholar]
  2. Abram J. W., Nedwell D. B. Inhibition of methanogenesis by sulphate reducing bacteria competing for transferred hydrogen. Arch Microbiol. 1978 Apr 27;117(1):89–92. doi: 10.1007/BF00689356. [DOI] [PubMed] [Google Scholar]
  3. Balderston W. L., Payne W. J. Inhibition of methanogenesis in salt marsh sediments and whole-cell suspensions of methanogenic bacteria by nitrogen oxides. Appl Environ Microbiol. 1976 Aug;32(2):264–269. doi: 10.1128/aem.32.2.264-269.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Braun M., Schoberth S., Gottschalk G. Enumeration of bacteria forming acetate from H2 and CO2 in anaerobic habitats. Arch Microbiol. 1979 Mar 12;120(3):201–204. doi: 10.1007/BF00423066. [DOI] [PubMed] [Google Scholar]
  5. Bryant M. P., Campbell L. L., Reddy C. A., Crabill M. R. Growth of desulfovibrio in lactate or ethanol media low in sulfate in association with H2-utilizing methanogenic bacteria. Appl Environ Microbiol. 1977 May;33(5):1162–1169. doi: 10.1128/aem.33.5.1162-1169.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. King G. M., Klug M. J. Comparative aspects of sulfur mineralization in sediments of a eutrophic lake basin. Appl Environ Microbiol. 1982 Jun;43(6):1406–1412. doi: 10.1128/aem.43.6.1406-1412.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Lovley D. R., Klug M. J. Intermediary metabolism of organic matter in the sediments of a eutrophic lake. Appl Environ Microbiol. 1982 Mar;43(3):552–560. doi: 10.1128/aem.43.3.552-560.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Mackie R. I., Bryant M. P. Metabolic Activity of Fatty Acid-Oxidizing Bacteria and the Contribution of Acetate, Propionate, Butyrate, and CO(2) to Methanogenesis in Cattle Waste at 40 and 60 degrees C. Appl Environ Microbiol. 1981 Jun;41(6):1363–1373. doi: 10.1128/aem.41.6.1363-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Mountfort D. O., Asher R. A., Mays E. L., Tiedje J. M. Carbon and electron flow in mud and sandflat intertidal sediments at delaware inlet, nelson, new zealand. Appl Environ Microbiol. 1980 Apr;39(4):686–694. doi: 10.1128/aem.39.4.686-694.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Mountfort D. O., Asher R. A. Role of sulfate reduction versus methanogenesis in terminal carbon flow in polluted intertidal sediment of waimea inlet, nelson, new zealand. Appl Environ Microbiol. 1981 Aug;42(2):252–258. doi: 10.1128/aem.42.2.252-258.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Smith R. L., Klug M. J. Electron donors utilized by sulfate-reducing bacteria in eutrophic lake sediments. Appl Environ Microbiol. 1981 Jul;42(1):116–121. doi: 10.1128/aem.42.1.116-121.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Smith R. L., Klug M. J. Reduction of sulfur compounds in the sediments of a eutrophic lake basin. Appl Environ Microbiol. 1981 May;41(5):1230–1237. doi: 10.1128/aem.41.5.1230-1237.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Strayer R. F., Tiedje J. M. Kinetic parameters of the conversion of methane precursors to methane in a hypereutrophic lake sediment. Appl Environ Microbiol. 1978 Aug;36(2):330–340. doi: 10.1128/aem.36.2.330-340.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Sørensen J., Christensen D., Jørgensen B. B. Volatile Fatty acids and hydrogen as substrates for sulfate-reducing bacteria in anaerobic marine sediment. Appl Environ Microbiol. 1981 Jul;42(1):5–11. doi: 10.1128/aem.42.1.5-11.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Winfrey M. R., Zeikus J. G. Effect of sulfate on carbon and electron flow during microbial methanogenesis in freshwater sediments. Appl Environ Microbiol. 1977 Feb;33(2):275–281. doi: 10.1128/aem.33.2.275-281.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]

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