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. Author manuscript; available in PMC: 2009 May 1.
Published in final edited form as: Biol Reprod. 2007 Dec 26;78(5):841–851. doi: 10.1095/biolreprod.107.065599

Fig. 4.

Fig. 4

Tissue-specific PGF expression is mediated by the region between −828 and −698 bp upstream of the PGF translation start site. JEG-3, JAR, hEK-293, HeLa, and MCF-7 cells were transfected with 1.5 μg of clone (−698/+34) or clone (−828/+34) and 0.5 μg of pRSVLuc. Cells were processed and fold activity above background plotted as in Figure 2. Clone (−828/+34) produced high promoter activity in trophoblast cells JEG-3 and JAR, but not in the nontrophoblast cells hEK-293 and HeLa. Clone (−698/+34) produced relatively similar activity in the various cell lines.