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Immunolocalization of Oct-4 (green) and F-actin (red) in the same morula and blastocyst stage embryos. A) Morulae and blastocysts were freshly collected from the reproductive tracts on days 3 and 4 of pregnancy. B) Morulae were obtained after electroporation of control and two dosages of ZO-1 siRNAs. The nuclei were stained with DAPI. Localization of these proteins is indicated with arrow head. TE, Trophectoderm; ICM, Inner Cell Mass.
