Table 1. Details of polymerase chain reaction primers for LIM2 amplification.
|
Exon |
Primer name |
Primer sequence (5′-3′) |
Product size (bp) |
Annealing Temp (°C) |
| I |
LIM 1F |
CCATTGTGTAGGGAGGCTTA |
213 |
52 |
| LIM 1R |
AGGTCCTGGGAGAAGAAGG |
|||
| II |
LIM 2AF |
CAGTTCCTCCCTTCAAGTCC |
159 |
53 |
| LIM 2AR |
ACTGCATCCAGTGGTCTGTT |
|||
| LIM 2BF |
TGTACAGCTTCATGGGTGGT |
255 |
52 |
|
| LIM 2BR |
TGGAATACAGGTGTCCTTGG |
|||
| LIM 2CF |
TACCTGCAGACAGACAGCAT |
238 |
52 |
|
| LIM 2CR |
CCCAACTTAACCTTCAAACC |
|||
| III |
LIM 3F |
TCATCTCAGAGGTAGCAGCA |
279 |
52 |
| LIM 3R |
ATTGGGGTTTGAGATGAGAG |
|||
| IV |
LIM 4F |
AAAATCACACCCAGCCTTAG |
248 |
51 |
| LIM 4R |
ACTCTATCTGCTGCCCACTC |
|||
| V |
LIM 5F |
GGTGTTGGGCTCTCTTG |
231 |
51 |
| LIM 5R | CTAGGAACCAGGATTTCA | |||
Sequences of forward (F) and reverse (R) primers used for amplification of LIM2 exons are shown above along with sizes of PCR products for each primer pair. Exon 2 was amplified in three fragments using primer sets denoted 2A-2C.