Skip to main content
. 2008 Jul;147(3):1380–1395. doi: 10.1104/pp.107.115436

Figure 2.

Figure 2.

Expression of the 35S:ARR1-SRDX gene fusion and of the ARR1 gene. A, Total RNA samples were isolated from 3-week-old plants, and the transcript was detected by northern-blot hybridization using a probe specific for the ARR1-SRDX gene fusion. The ACTIN2 transcript was used as a loading control. WT, Wild-type Col-0; ARR1-S-8 and ARR1-S-10 are independent lines of 35S:ARR1-SRDX transgenic plants. B, Analysis of ARR1 transcript levels. Total RNA was isolated from 5-d-old wild-type and 35S:ARR1-SRDX transgenic seedlings. Northern blots were hybridized with a probe specific for that C-terminal part of ARR1 that had been deleted during the construction of the 35S:ARR1-SRDX gene and thus hybridizes only with the native ARR1 and not with the ARR1-SRDX fusion transcript. Total RNA was used as a loading control.