Table I.
Substrate specificities of AtMES proteins
AtMES family members (AtMES1–AtMES20) are listed with the respective gene identification numbers. Fifteen heterologously expressed AtMES proteins were assayed for esterase activities with PNPA, MeIAA, MeSA, MeJA, MeGA4, and MeGA9, as described in “Materials and Methods.” +, Active; −, not active; n.d., not determined.
| Name | Gene ID | PNPA | MeIAA | MeSA | MeJA | MeGA4 | MeGA9 |
|---|---|---|---|---|---|---|---|
| AtMES1 | At2g23620 | + | + | + | + | − | − |
| AtMES2 | At2g23600 | + | + | + | + | − | − |
| AtMES3 | At2g23610 | + | + | − | + | − | − |
| AtMES4 | At2g23580 | + | − | + | − | − | − |
| AtMES5 | At5g10300 | − | − | − | − | − | − |
| AtMES6 | At2g23550 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| AtMES7 | At2g23560 | + | + | + | − | − | − |
| AtMES8 | At2g23590 | + | − | − | − | − | − |
| AtMES9 | At4g37150 | + | + | + | + | − | − |
| AtMES10 | At3g50440 | − | − | − | + | − | − |
| AtMES11 | At3g29770 | − | − | − | − | − | − |
| AtMES12 | At4g09900 | − | − | − | − | − | − |
| AtMES13 | At1g26360 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| AtMES14 | At1g33990 | − | − | − | − | − | − |
| AtMES15 | At1g69240 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| AtMES16 | At4g16690 | + | + | − | + | − | − |
| AtMES17 | At3g10870 | + | + | − | − | − | − |
| AtMES18 | At5g58310 | − | + | − | − | − | − |
| AtMES19 | At2g23570 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |
| AtMES20 | At4g37140 | n.d. | n.d. | n.d. | n.d. | n.d. | n.d. |