Figure 2.

Degradation of lotaustralin, linamarin, and rhodiocyanosides in leaves of L. japonicus following cell disruption. The rate of degradation of endogenous hydroxynitrile glucosides in transgenic L. japonicus 35S∷CYP79D2 leaves following tissue disruption was monitored by LC-MS and analysis of the amounts of metabolites remaining at time points between 0 and 60 min following cell disruption. The total amount of hydroxynitrile glucosides (linamarin + lotaustralin + rhodiocyanosides) at time 0 was defined as 100. Rhodiocyanosides are most rapidly degraded, followed by lotaustralin, which is degraded at a moderate rate; only negligible degradation of linamarin is observed.