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Substrate specificity profiles of LjBGD2 and LjBGD4 expressed in Arabidopsis. Arabidopsis leaf discs producing recombinant LjBGD2 and LjBGD4 were assayed for the ability to hydrolyze a range of β-glycosides. Rhod A, Rhodiocyanoside A. All incubation assays (10 min) were conducted using 1 mm substrate corresponding to a total of 200 nmol. Arabidopsis wild-type leaf discs served as a negative control.
