Table I.
Selected seed-related genes up-regulated in brm-5 leaves as revealed by microarray analysis
| Gene Identification | Locus | Fold Elevateda |
|---|---|---|
| SSP genes | ||
| 2S seed storage protein 2 (At2S2) | At4g27150b | 59.8 |
| At2S3 | At4g27160b | 10.1 |
| At2S5 | At5g54740b | 8.2 |
| Cupin family protein (At7S1) | At4g36700b | 120.9 |
| Other storage protein genes | ||
| LTP | At5g38160b | 4.5 |
| LTP2 | At2g38530b | 3.0 |
| LTP | At1g62500b | 2.7 |
| LTP | At5g48490b | 2.6 |
| LTP6 | At3g08770b | 2.0 |
| Lipoxygenase (LOX2) | At3g45140 | 3.6 |
| Em-like protein GEA1 (Em1) | At3g51810b | 9.4 |
| EMB genesc | ||
| Oligopeptide transporter OPT family protein (AtOPT3) | At4g16370b | 7.7 |
| Zinc finger protein-related/E3 ligase (EMB2454) | At3g18290b | 5.5 |
| RNA polymerase σ-subunit SigE (SIG5) | At5g24120b | 2.3 |
| DNA-directed DNA polymerase ɛ catalytic subunit (POL2B/TIL2) | At2g27120 | 19.0 |
| Metallo-β-lactamase family protein (EMB2746) | At5g63420 | 2.4 |
a
Fold change is relative to wild type (βCGpro:GUS).
b
Genes for which the elevated expression was validated by RNA-blot or PCR analyses (see Fig. 3).
c
Essential genes required for embryogenesis (Tzafrir et al., 2004; http://www.seedgenes.org).