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. 2008 Jul;147(3):1325–1333. doi: 10.1104/pp.108.117168

Table I.

Primers used in this study

The restriction sites used for cloning are underlined.

Primers Used
Degenerate primers for cloning of CpFAH
    DM34: 5′-GCICAYGARTGYGGICAYSRIGCITT-3′
    DM36: 5′-TAIGTDATIGCIACIARCCARTGRTKIACCCA-3′
For 5′ and 3′ RACE experiments
    DM58: 5′-GGAGCGCGACATGGTCTTTCTGCCTCAG-3′
    DM59: 5′-CAGACAGCCATGTTGGCCCAGCCGAAT-3′
For full-length cDNA amplification
    DM61: 5′-CACTAGGGCAACGAATTACTCTGC-3′
    DM62: 5′-GGACGCCATCGTTGACTTCC-3′
For yeast and plant expression
    DM63: 5′-GCGAATTCGAAATGGCTTCCGCTACTCC-3′
    DM64: 5′-GCGAATTCCTACTGAGTCTTCATTGAAATGG-3′
    DM68: 5′-TCTAGAGAAATGGCTTCCGCTACTCC-3′
    DM69: 5′-TCTAGACTACTGAGTCTTCATTG-3′
    DM81: 5′-CGCGGATCCATGGCCTCCTCCGAGAAC-3′
    DM82: 5′-CGCGGATCCTCACAGGAACAGGTGGTGGC-3′