Degenerate primers for cloning of CpFAH
|
DM34: 5′-GCICAYGARTGYGGICAYSRIGCITT-3′ |
DM36: 5′-TAIGTDATIGCIACIARCCARTGRTKIACCCA-3′ |
For 5′ and 3′ RACE experiments |
DM58: 5′-GGAGCGCGACATGGTCTTTCTGCCTCAG-3′ |
DM59: 5′-CAGACAGCCATGTTGGCCCAGCCGAAT-3′ |
For full-length cDNA amplification |
DM61: 5′-CACTAGGGCAACGAATTACTCTGC-3′ |
DM62: 5′-GGACGCCATCGTTGACTTCC-3′ |
For yeast and plant expression |
DM63: 5′-GCGAATTCGAAATGGCTTCCGCTACTCC-3′ |
DM64: 5′-GCGAATTCCTACTGAGTCTTCATTGAAATGG-3′ |
DM68: 5′-TCTAGAGAAATGGCTTCCGCTACTCC-3′ |
DM69: 5′-TCTAGACTACTGAGTCTTCATTG-3′ |
DM81: 5′-CGCGGATCCATGGCCTCCTCCGAGAAC-3′ |
DM82: 5′-CGCGGATCCTCACAGGAACAGGTGGTGGC-3′ |