Table II.
Fatty acid composition of single seeds of Arabidopsis wild-type (WT) and mutant (fad2/fae1) transgenics
The values are derived from GC analysis of FAME-TMS derivatives (mean ± se).
| Fatty Acids | WT
|
fad2/fae1
|
||
|---|---|---|---|---|
| Nontransformed | CpFAH | Nontransformed | CpFAH | |
| % | % | % | % | |
| 16:0 | 8.35 ± 0.56 | 10.38 ± 0.75 | 5.6 ± 0.23 | 4.18 ± 0.48 |
| 16:1 | 1.04 ± 0.77 | 0.07 ± 0.05 | – | – |
| 18:0 | 5.12 ± 0.55 | 5.82 ± 0.54 | 3.71 ± 0.21 | 6.56 ± 0.30 |
| 18:1-9 | 10.79 ± 0.32 | 13.76 ± 1.46 | 81.62 ± 0.56 | 45.04 ± 1.22 |
| 18:1-11 | 5.14 ± 0.86 | 5.48 ± 0.90 | 3.24 ± 0.05 | 5.51 ± 0.27 |
| 18:2-9,12 | 25.50 ± 0.54 | 24.69 ± 0.72 | 1.58 ± 0.34 | 8.96 ± 0.23 |
| 18:3-9,12,15 | 15.97 ± 0.74 | 5.22 ± 0.24 | 2.48 ± 0.06 | 3.85 ± 0.06 |
| 20:0 | 2.55 ± 0.06 | 1.43 ± 0.08 | 1.08 ± 0.02 | 0.3 ± 0.02 |
| 20:1-11 | 19.97 ± 0.80 | 10.16 ± 0.36 | 0.70 ± 0.02 | 0.2 ± 0.01 |
| 20:1-13 | 2.17 ± 0.05 | 2.62 ± 0.24 | – | – |
| 20:2 | 1.90 ± 0.08 | 0.51 ± 0.10 | – | – |
| 22:1 | 1.68 ± 0.13 | 0.23 ± 0.08 | – | – |
| 12-OH-18:1-9 | 0 | 12.65 ± 0.41 | – | 17.86 ± 1.09 |
| 12-OH-18:2-9,15 | 0 | 3.43 ± 0.32 | – | 7.54 ± 0.39 |
| 14-OH-20:1-11 | 0 | 3.42 ± 0.24 | – | – |
| 14-OH-20:2-11,17 | 0 | 0.15 ± 0.05 | – | – |
| Total hydroxyl fatty acids | 0 | 19.65 ± 0.73 | – | 25.00 ± 1.43 |