The expression levels of the transgene and galactinol and raffinose contents in the leaves of Ox-GolS1 and Ox-GolS2 plants. Four-week-old wild-type, Ox-GolS1-11, Ox-GolS2-8, and Ox-GolS2-29 plants grown under control growth conditions were used for the analysis. The procedures are described in “Materials and Methods.” A, The transcript level of GolS1 or GolS2. Relative amounts were normalized to Actin2 mRNA (100%). B, Total GolS activities. C, Galactinol and raffinose contents. Mean ± sd values from three experiments are shown. Asterisks indicate that the values are significantly different from those in the wild-type plants (P < 0.05).