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. Author manuscript; available in PMC: 2009 Jun 15.
Published in final edited form as: Anal Biochem. 2008 Mar 15;377(2):251–258. doi: 10.1016/j.ab.2008.03.018

Fig. 2.

Fig. 2

Comparison of sugar indicator sensitivity, specificity and cross reactivity. (A) The absorbance responses of six reducing sugar assays (tetrazolium blue, ▲; ferricyanide, ■; Honda MBTH, ○ Anthon MBTH, △; pHBH, □) toward varying amounts of glucose. (B) The absorbance response of the DNS assay (●), in comparison to tetrazolium blue (▲) and pHBH (□), toward varying amounts of glucose. (C) Cross reactivity of assays toward representative sugars (Glc, glucose; GlcN, glucosamine; dGlc, 2-deoxyglucose) and glycosides (UDP-Glc uridine 5′diphospho-α-D-glucose; GlcOMe, methyl-α-D-glucopyranoside, Glc-1-P, α-D-glucose-1-phosphate). Substrate amounts for each indicator were adjusted to be equivalent to the amount of glucose required for a 1 AU response (DNS, 2500 nmol; tetrazolium blue, 250 nmol; terricyanide, 7.5 nmol; Honda MBTH, 50 nmol; Anthon MBTH, 50 nmol; pHBH, 100 nmol). (D) Cross reactivity with BSA (tetrazolium blue, ▲; ferricyanide, ■; Honda MBTH, ○; Anthon MBTH, △; pHBH, □; DNS, ●). The x-axis (BSA) is plotted on a logarithmic scale. Turbidity was observed with the tetrazolium blue and Anthon MBTH assays at high levels of protein.