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. Author manuscript; available in PMC: 2009 Mar 1.
Published in final edited form as: Invest Ophthalmol Vis Sci. 2008 Mar;49(3):1037–1047. doi: 10.1167/iovs.07-1279

FIGURE 4.

FIGURE 4

Differential effects of FK506 on FKBP51-chaperoned nuclear translocation of GRβ in normal NTM-5 and glaucomatous GTM-3 cells. Confluent NTM-5 and GTM-3 cells were pretreated with vehicle or 1 µM FK506 for 1 hour and then subjected to additional vehicle control or 100 nM DEX treatment, as labeled, for another 30 minutes. The cytosol (100 µg) and nuclear (50 µg) fraction proteins were prepared and resolved in 4% to 15% gradient SDS-polyacrylamide gels after immunoprecipitation. (A) Coimmunoprecipitation with anti-FKBP52 antibody followed by Western immunoblot analysis to detect GRα in NTM-5 cells. (B) Coimmunoprecipitation with anti-FKBP51 antibody followed by Western immunoblot analysis to detect GRβ in NTM-5 cells. (C) Coimmunoprecipitation with anti-FKBP51 antibody followed by Western immunoblot analysis to detect GRβ in GTM-3 cells. The presented data are representative of the results of three independent experiments.