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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Biochim Biophys Acta. 2008 Mar 18;1782(6):421–425. doi: 10.1016/j.bbadis.2008.03.004

Figure 5. Inhibition of ALDH activity by nonanal and 4-HNE.

Figure 5

A, RT-PCR analysis of ALDH1A1 and ALDH1A2 expression in HEK293 cells. MW st., molecular weight standards. B, Purified human ALDH1A1 was incubated with retinaldehyde (5 µM) and NAD+ (1 mM) in the presence of 0–200 µM nonanal or 4-HNE. The amount of produced retinoic acid was determined by HPLC as described under Materials and Methods. Data shown are representative of three independent experiments.