Table 1.

Expression of mNek1 truncations disrupt centrosomal γ-tubulin.

mNek1 aa residues expressed with GFP tag	Subcellular localization of GFP-truncated Nek1	Percent of transfected cells without γ-tubulin foci
1–258	Cytoplasmic	0
1–352	Nuclear	0
1–407	Nuclear	8 ± 1
1–510	Nuclear	68 ± 3
1–599	Nuclear	64 ± 3
1–686	Nuclear	64 ± 4
1–686 (K33R)	Ciliary	0

Quantification of the loss of γ-tubulin foci is based on examination of 100 transfected (green) cells from each of three independent experiments. These were scored as either retaining or lacking detectable γ-tubulin foci. Shown is the average number of transfected cells that lack γ-tubulin foci, plus or minus the standard deviation. Constructs containing at least the first two coiled-coils significantly reduce the number of transfected cells retaining γ-tubulin foci (constructs 1–510 and 1–599).