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Denaturing PAGE of [32P]FUra-tRNAs and complexes with ΨSI. (A) SDS/PAGE of FUra-tRNA (lane 1), ΨSI-FUra-tRNA covalent complex (lane 2), and RNase A digest of ΨSI-FUra-tRNA covalent complex from lane 2 (lane 3). (B) 7 M urea-15% PAGE of unmodified (lane 1), ΨSI-modified FUra-tRNA (lane 2), and the oligo containing FU39 after hydrolysis of the RNase A-digested ΨSI-FUra-tRNA complex from A, lane 3.
