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7 M urea-PAGE of partial RNase A digests of unmodified (lane 2) and ΨSI-modified FUra-tRNA (lane 1). FUra-tRNA was labeled with [32P]pCp, and the location of the oligo containing modified FU39 (arrow) was determined by comparison to sequence ladders from RNase T1 and T2 digests (data not shown).
