Figure 5.

TLC of standards and radioactive nucleosides derived from digestion of ΨSI-modified [6-³H]FUra-tRNA. (Lane 1) [6-³H]5-FUrd; (lane 2) photoproducts of [6-³H]5-FUrd; (lane 3) HPLC-purified photoproduct 1 of [6-³H]5-FUrd; (lane 4) HPLC-purified photoproduct 2 of [6-³H]5-FUrd; (lane 5) nuclease P1 and alkaline phosphatase digests of ΨSI-modified [6-³H]FUra-tRNA; (lane 6) nuclease P1 and alkaline phosphatase digests of [6-³H]FUra-tRNA. Lanes 5 and 6 migrate aberrantly because of the high salt concentration; for clarity, the components of each lane are segregated by vertical lines.