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. 2008 Jul 7;205(7):1601–1610. doi: 10.1084/jem.20080091

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© 2008 Kato et al.

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Figure 4. — RIG-I and MDA5 selectively recognize dsRNA in a length-dependent manner. (A and B) Indicated genotype of MEFs were treated with 1 μg/ml of the indicated RNAs (10 μg/ml in the case of high dose) for 24 h. s, sense ssRNA; as, antisense ssRNA; s+as, dsRNA generated by annealing s with as. s and as are chemically synthesized 70-bp ssRNAs having a 5′ hydroxyl end in A. s and as are in vitro transcribed capped-ssRNAs in (B). The production of IFN-β in the supernatant was measured by ELISA. (C) WT, Rig-I^−/−, Mda5^−/−, and Rig-I^−/−Mda5^−/− MEFs were treated with 1 μg/ml of in vitro–transcribed capped dsRNAs for 16 h. The production of IFN-β in the supernatant was measured by ELISA. Error bars show SDs between triplicates.