Figure 1.

Pulmonary DC dynamics during influenza virus infection. (A) Mice were infected with a sublethal dose of influenza and killed at the indicated days a.i. Total numbers of CD11c⁺MHC II⁺ DCs in the lungs were determined by flow cytometry. n = 5–9 mice/time point. Data are representative of two to three independent experiments. (B) Mice were administered i.n. CFSE and infected 6 h later. Mice were killed at the indicated times a.i., and the frequency of CFSE⁺ rDCs among total CD11c⁺MHC II⁺ LN resident (LN DC) in the lung draining LN was determined by flow cytometry. (C) Mice were infected with influenza, and then administered clodronate-liposomes (black bars, aDC depleted) or PBS-liposomes (gray bars) i.n. at 48 h a.i. Untreated mice served as an influenza-infected control (white bars). 6 h after treatment, lungs were examined by flow cytometry for the frequency (left) and total numbers (right) of aDCs (CD11c⁺CD11b⁻MHC II⁺Autofluorescence^neg) and iDCs (CD11c⁺CD11b⁺MHC II⁺). The data are the mean values ± the SEM (n = 4 mice/group) and are representative of two separate experiments. Clodronate-liposome depletion of aMφ is shown in Fig. S1. Fig. S1 is available at http://www.jem.org/cgi/content/full/jem.20080314/DC1.