Figure 3.

aDC depletion at 48 h a.i. impairs pulmonary influenza-specific CD8 T cell responses. Mice were infected with influenza and aDC depleted as in Fig 1. The gating strategy for tetramer⁺ or IFN-γ⁺ cells among CD8a⁺ cells is shown in A. On day 6 a.i., lungs were harvested and analyzed by flow cytometry for total number (B) of influenza-specific tetramer⁺ or IFN-γ⁺ CD8 T cells. Control influenza infected (white bars) and naive (dark gray bars) mice were included as controls. The data are the mean values ± the SEM (n = 8–11 mice/group) and are representative of two to four separate experiments. In A, representative plots for individual mice are shown. (C) On day 6 a.i., in vivo cytotoxicity assays were performed on aDC-depleted (shaded bars) and control (white bars) mice. Samples were examined by flow cytometry for the percentage of specific lysis. The data are the mean values ± the SEM (n = 6–7 mice/group) and are representative of two separate experiments.