Table 4.

Gene and EST-based marker primer sequences. Primers used for DNA amplification in the markers obtained from Fragaria gene or EST or Prunus EST sequences.

Origin	Locus name	Forward primer sequence 5' – 3'	Reverse primer sequence 5' – 3'	Referencea
Fragaria gene	EKO	ACAGTCCAGCTCCAATAGTTCC	GCTTTCCCATTGATTCTTGTCC	AY462247
	F3H	GAGTTGATACCAAGCTCATCTCG	GTCACCTCTCTCCATCCTTCC	AB097151
	DFR	CCACTCCTATGGATTTTGAGTCC	CTAGCACCCCATTTATTGTTGG	AB095030
	ADH	GCKTCAMGAATTATYGGKGTTG	ATGGGASTTKRTGGGTGATG	X15588
Fragaria EST	EFvVB2179	ATCTGCGTGACAATGCAAAG	AAGAGCCTTCAGTTGCTCCA	CX662179
	EFvVB2119	GCTCGAGCTGATTACGATTACC	TAAAGGACCCATCAGAGAAACG	CX662119
	EFvNH8894	GGTTAGGTCTCCCCAGTGGT	GGACGTCTCCCACTAGCATC	DV438894
	EFvVB1231	CCAACTGTGACATCCACGAC	GCTGTCACGCAGAAAATCAA	CX661231
	EFaUF6868	GCTCTTCCAGGTCGAGTACG	GTTTCCACTTGGGCAGTTGT	CO816868
	EFaTR1976	GTTGGTGCTGAGTTTGGTGA	CCCAACTGCTCAAGAAGGAG	CO381976
	EFaUF7699	GTTCTTGTTGGTGGAAGCAC	CCTCAAAGACCTGAATGGAG	CO817699
	EFaUF7084	CAGAAGAGGTTCAAGTTCC	ACACCATAGCAAGCCCTG	CO817084
	EFvNH8484	TTCTGGTGTCGGCAAGTC	AGGCCTGCTCAACATTGG	DV438484
	EFvVB2013	GTGCAGTTGCCAAAGGAGC	AGCTGGGTTTGCTGCTT	CX662013
	EFvNH9852	TTCTGTCGTGGTGTCCC	ATGATCTTTTGGCGACCA	DV439852
	EFvNH7822	GATGCTGGGTCTGCTGGG	GCCTGCTCATTGGCATA	CO817822
	EFaUF7248	ACTGCTCGCCCAATGAAG	TCACATCAGCATCCATGTCA	CO817248
	EFavB1923	GGCCGTGTCTCTTGCAGT	TGGGAGCAAATCCACCTT	CX661923
	ACO	AGCACCTTCTACCTCAAACACC	CTCACAGAACAAGTCCAAGAGC	AF129073
Prunus EST	EPpCU9642	TTCAGTTGGCAGATCCTGTG	TGCTGAGACCCTTCCAATTT	BU039642
	EPpCU1785	TTTTCCAAACCTTGCTGGAG	GCAGTAGCTGTGGCAATGAA	BU041785
	EPpCU7308	GGCAGGCCGCTCTTATACTA	GACTCTTTTCGGGGTTCCA	BU047308
	EPpCU9257	CACCACCGTTTCAAAAGAGG	CTGAAGCTCTAGCTGAGGCAAG	BU039257
	EPpCU1830	TGATGCAATTGGCACAAAGC	CCTATCACCACTTACTTCACTGC	BU041830
	EPpCU9910	ATAACTCTGCCATCCGAATCC	CATTCCTTGAACAGATCCTTGC	BU039910
	EPpCU9223	AACAGAGCCAAGCTTATGCAG	TTTCTGCGCAACCGCATC	BU039223
	EPpCU2875	AACTCAGAGACATATCTGCACAGG	AAGTTGAAGCGGTCTTCATAGG	BU042875

^aEMBL accession numbers for the sequences from which novel primer pairs were designed