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. Author manuscript; available in PMC: 2009 Jun 1.
Published in final edited form as: Bone. 2008 Feb 15;42(6):1072–1079. doi: 10.1016/j.bone.2008.01.023

Figure 7. M180 mRNA expression is partly mediated by NO signaling activated by the NOS2 isozyme.

Figure 7

LS8 cells, cultured in 2% FBS-DMEM, were treated with 10 ng/ml r[A−4] for 4 h. Panel A: total nitrites were quantitated spectrophotometrically with the Griess reagent, analyzed by linear regression, and expressed as µmoles/mg cellular protein. To confirm NOS2-mediated production of NO, LS8 cells were pre-treated with 0.5 mM L-Nil, followed by 10 ng/ml r[A−4]. Panel B: the expression of M180 mRNA was analyzed by semi-quantitative real-time PCR, and target mRNA expression was normalized to GAPDH mRNA expression, according to the ΔCt method. The histogram depicts one representative change in M180 expression (expressed as %) in the sample treated with r[A−4] vs. the sample co-treated with L-Nil and r[A−4].