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. 2008 May 13;112(2):398–405. doi: 10.1182/blood-2007-12-126714

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© 2008 by The American Society of Hematology

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Increased workers of endothelial damage in sph/sph mice. Plasma levels of sVCAM-1 (A), sP-sel (C), and sE-sel (E) are increased in sph/sph mice compared with control mice. Levels of soluble murine vascular cell adhesion molecule-1 (sVCAM-1, A), soluble P-selectin (sP-sel, C), and soluble E-selectin (sE-sel, E) were determined by ELISA of plasma samples from control wild-type (+/+, □), control heterozygote (sph/+, ), and sph/sph (■) mice. Data are presented as mean (± SEM) for the following numbers: (A) n = 7 +/+ [all more than 8 weeks of age], n = 7 sph/+[all more than 8 weeks of age], n = 6 sph/sph [all more than 8 weeks of age]; (C,E) n = 13 +/+ [n = 6, 6-8 weeks of age; n = 7, more than 8 weeks of age], n = 12 sph/+[n = 6, 6-8 weeks of age; n = 6, more than 8 weeks of age], n = 17 sph/sph [n = 9, 6-8 weeks of age; n = 8, more than 8 weeks of age]. No significant difference between age groups was noted for sP-sel and sE-sel values. *Significant difference between sph/sph and control (+/+, sph/+) values: P < .0001 for sVCAM-1 (A) and sP-sel (C); P < .005 for sE-sel (E). Levels of sVCAM-1 (B), sP-sel (D), and sE-sel (F) in the plasma of sph/sph and control mice are variably reduced by ultracentrifugation. Plasma from +/+ (□ and gray-striped bars) mice more than 6 weeks of age and sph/sph mice (black and black-striped bars) more than 8 weeks of age was subjected to ultracentrifugation to remove microparticles as described under “Methods.” Levels of sVCAM-1 (B), sP-sel (D), and sE-sel (F) in plasma were compared before (“−UCS,” open and black bars) and after (“+UCS,” gray-striped and black-striped bars) ultracentrifugation. The difference between sVCAM-1 levels in A and B and sP-sel levels in C and D reflects the fact that plasma samples from separate sets of +/+ and sph/sph mice were used for the assays. Data are presented as mean (± SEM) for the following numbers: (B) n = 4 +/+ and n = 3 sph/sph; (D) n = 3 +/+ and n = 3 sph/sph; (F) n = 3 +/+ and n = 4 sph/sph. *Significant difference between “−UCS” and “+UCS” values, P < .03.

Inline graphic — Increased workers of endothelial damage in sph/sph mice. Plasma levels of sVCAM-1 (A), sP-sel (C), and sE-sel (E) are increased in sph/sph mice compared with control mice. Levels of soluble murine vascular cell adhesion molecule-1 (sVCAM-1, A), soluble P-selectin (sP-sel, C), and soluble E-selectin (sE-sel, E) were determined by ELISA of plasma samples from control wild-type (+/+, □), control heterozygote (sph/+, ), and sph/sph (■) mice. Data are presented as mean (± SEM) for the following numbers: (A) n = 7 +/+ [all more than 8 weeks of age], n = 7 sph/+[all more than 8 weeks of age], n = 6 sph/sph [all more than 8 weeks of age]; (C,E) n = 13 +/+ [n = 6, 6-8 weeks of age; n = 7, more than 8 weeks of age], n = 12 sph/+[n = 6, 6-8 weeks of age; n = 6, more than 8 weeks of age], n = 17 sph/sph [n = 9, 6-8 weeks of age; n = 8, more than 8 weeks of age]. No significant difference between age groups was noted for sP-sel and sE-sel values. *Significant difference between sph/sph and control (+/+, sph/+) values: P < .0001 for sVCAM-1 (A) and sP-sel (C); P < .005 for sE-sel (E). Levels of sVCAM-1 (B), sP-sel (D), and sE-sel (F) in the plasma of sph/sph and control mice are variably reduced by ultracentrifugation. Plasma from +/+ (□ and gray-striped bars) mice more than 6 weeks of age and sph/sph mice (black and black-striped bars) more than 8 weeks of age was subjected to ultracentrifugation to remove microparticles as described under “Methods.” Levels of sVCAM-1 (B), sP-sel (D), and sE-sel (F) in plasma were compared before (“−UCS,” open and black bars) and after (“+UCS,” gray-striped and black-striped bars) ultracentrifugation. The difference between sVCAM-1 levels in A and B and sP-sel levels in C and D reflects the fact that plasma samples from separate sets of +/+ and sph/sph mice were used for the assays. Data are presented as mean (± SEM) for the following numbers: (B) n = 4 +/+ and n = 3 sph/sph; (D) n = 3 +/+ and n = 3 sph/sph; (F) n = 3 +/+ and n = 4 sph/sph. *Significant difference between “−UCS” and “+UCS” values, P < .03.