. Author manuscript; available in PMC: 2008 Jul 2.

Published in final edited form as: Drug Metab Dispos. 2007 Mar 7;35(6):973–980. doi: 10.1124/dmd.107.014779

TABLE 3.

CBR1 V88 and CBR1 I88 kinetic constants for prototypical substrates

Substrate	CBR1 V88				CBR1 I88
Substrate	K_m	V_max	K_cat	V_max/K_m	K_m	V_max	K_cat	V_max/K_m
	μM	nmol/min · mg	S⁻¹	mg/min × 10⁻³	μM	nmol/min · mg	s⁻¹	mg/min × 10⁻³
Menadione^a	42 ± 23	220 ± 27	110	5.20	55 ± 21	168 ± 16	85	3.06
Daunorubicin^a	173 ± 26	181 ± 13	91	1.04	140 ± 23	121 ± 12	61	0.90
PGE₂^a	309 ± 94	53 ± 7	25	0.17	320 ± 63	35 ± 4	18	0.11
NADPH^b	247 ± 29	356 ± 30	179	1.44	343 ± 63	295 ± 31	149	0.86

Values represent the mean ± S.D. from four experiments performed in duplicates with two independent protein preparations.

Menadione was held constant at 250 μM. Values represent the mean ± S.D. from two experiments performed in duplicates with two independent protein preparations.