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Glutamate uptake activity of wild-type and cysteine-less GltT. Glutamate uptake in right-side-out membrane vesicles prepared from E. coli cells expressing His-tagged wild-type GltT (■), cysteine-less GltT (▴), or no GltT (●). (Inset) Expression levels of the wild-type and cysteine-less mutant on a Western blot stained with antibodies against the His-tag.
