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Western blot analysis of maleimide-labeled and purified GltT mutants S129C and Q412C. Western blots were decorated with streptavidin coupled to alkaline phosphatase to detect biotinylated proteins (A) and antibodies against the His-tag to compare the amounts of protein (B). Single-cysteine mutants S129C and Q412C were labeled with biotin maleimide with (+) or without (−) prior incubation with AMdiS, as indicated, in whole cells (lanes 1–4) and inside-out membrane vesicles (lanes 5–8).
