Fig. 3.

Conversion is regulated by DC maturation status. (A) Conversion cultures with naïve OTII CD4 T cells (CD25⁻Foxp3GFP⁻) and splenic DC subsets were set up as before in the presence of TGF-β (2 ng/ml) and IL-2 (50 units/ml). Antibodies (αCD40 or αCD154, 5 ng/ml) or LPS (5 ng/ml) were added as indicated. (B and C) DC subsets were purified from CD40^−/− mice (B) or CD80/86^−/− mice (C) and used in the conversion assay. The percentage of Foxp3⁺ cells was analyzed on day 5 and plotted. All conditions were performed in duplicate wells and reported as means ± SEM. Shown are representative results of three independent experiments.