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. Author manuscript; available in PMC: 2008 Jul 2.
Published in final edited form as: Biochemistry. 2007 Feb 27;46(12):3734–3744. doi: 10.1021/bi062214k

Table 2.

Steady-State Kinetics Parameters of Ligation with Gh•G and T•G on the 5′end of the Nick

Km(μM) kcat(min−1) kcat/Km(min −1μM−1)
Gh·G 1.5 ± 0.1 0.45 ± 0.02 0.31
T·G 0.055 ± 11 0.015 ± 0.001 0.28