Table 2.

Splice variants of PMCAs expressed in OSNs

Isoform	A site	C site
PMCA1	X, N = 3	b, N = 3
PMCA2	NA	b, N = 5
PMCA3	NA	b, N = 5
PMCA4	X, N = 3	a, N = 5

NA, not yet determined; PMCA1AX, a 39-nucleotide intron remains within the sequence; PMCA1Cb, the entire 159-nucleotide intron is removed from the splice site; PMCA2Cb, both the 172-nucleotide intron and the 55-nucleotide intron are removed from the sequence; PMCA3Cb, a small 68-nucleotide intron and a 154-nucleotide intron are completely removed; PMCA4Ax, a 36-nucleotide intron remains in the finished sequence; and PMCA4Ca, a 175-nucleotide intron is excised from the final product. Splice variants of the four isoforms of PMCAs expressed in OSNs were determined by sequencing PCR products of cDNA from FACS sorted OSNs that express GFP. The PCR primers were chosen to amplify the regions of the variable splicing in the 5′ and 3′ portions of the cDNAs.