. 2008 Jul 16;3(7):e2703. doi: 10.1371/journal.pone.0002703

Table 1. Different iVα14 NKT subsets in total lymphocytes of thymus, spleen and liver in WT, B7-1/2 and CD28 deficient mice.

		WT	B7KO	CD28KO
Thymus	NK1.1⁻CD4⁺	0.05±0.02	0.06±0.01	0.06±0.01
	NK1.1⁺CD4⁺	0.52±0.23	0.13±0.02^*	0.03±0.01^*
	NK1.1⁺CD4⁻	0.3±0.15	0.03±0.01^*	0.03±0.01^*
Spleen	NK1.1⁻CD4⁺	0.21±0.04	0.22±0.05	0.16±0.03
	NK1.1⁺CD4⁺	0.53±0.07	0.23±0.06^**	0.13±0.05^***
	NK1.1⁺CD4⁻	0.1±0.01	0.03±0.01^***	0.02±0.01^***
Liver	NK1.1⁻CD4⁺	0.48±0.26	1.21±0.29^*	0.68±0.21
	NK1.1⁺CD4⁺	1.34±0.44	1.46±0.33	0.64±0.19^*
	NK1.1⁺CD4⁻	0.72±0.26	0.3±0.05^*	0.21±0.05^*

Lymphocytes from thymus, spleen and liver of age and sex-matched WT, B7-1(-/-)/B7-2(-/-) or CD28(-/-) mice were analyzed by flow cytometry. The iVα14 NKT cells were identified based on expressing TCRβ and binding to the α-Galcer/CD1d tetramer. Data shown are means and SD of the percentage of given subsets in total viable lymphocytes (n = 4). Similar data were obtained in another experiment involving 5 mice per group.

P<0.05,

^**

P<0.01,

^***

P<0.001.