Figure 1.
15d-PGJ2 inhibits IL-1β expression by Aβ1−42 plus LPS-activated microglia. (a) Primary mouse microglial cells were pretreated for 1 hour with 15d-PGJ2 (2.5 μM). Aβ 1−42 (5 μM), LPS (10 ng/mL), or Aβ 1−42 (5 μM) plus LPS (10 ng/mL) was added as indicated, and 24 hours later, the concentration of IL-1β in the culture medium was determined. Values represent the mean ± s.e.m for a representative experiment run in triplicate. At least three independent experiments were conducted. (b) Cells were pretreated for 1 hour with 15d-PGJ2 (2.5 μM). Aβ 1−42 (10 μM), LPS (5 ng/mL), or Aβ 1−42 (10 μM) plus LPS (5 ng/mL) was added as indicated, and 6 hours later, total RNA was isolated. IL-1β mRNA levels were determined by real-time quantitative RT-PCR. Results are expressed as fold inductions in GAPDH normalized mRNA values versus levels in LPS-treated cells. Values are mean ± s.e.m of six samples derived from three independent experiments, with each experiment performed in duplicate. **P < .01 and ***P < .001 versus Aβ 1−42 + LPS-treated cultures.