Figure 3.
15d-PGJ2 inhibits microglial mRNA expression of IL-12p40, IL-12p35, and IL-23p19 induced by Aβ1−42 plus LPS. Cells were pretreated for 1 hour with 15d-PGJ2 (2.5 μM). Aβ 1−42 (10 μM), LPS (5 ng/mL), or Aβ 1−42 (10 μM) plus LPS (5 ng/mL) was added as indicated, and 6 hours later, total RNA was isolated. IL-12p35 (a), IL-12p40 (b), and IL-23p19 (c) mRNA levels were determined by real-time quantitative RT-PCR. Results are expressed as fold inductions in GAPDH normalized mRNA values versus levels in LPS-treated cells. Values are mean ± s.e.m of six samples derived from three independent experiments, with each experiment performed in duplicate. *P < .05, **P < .01, and ***P < .001 versus Aβ 1−42 + LPS-treated cultures.