The effects of 15d-PGJ2 on microglial mRNA expression of CD14,
MyD88, TLR4, and TLR2 induced by Aβ1−42
plus LPS. Cells were pretreated for 1 hour with 15d-PGJ2 (2.5 μM). Aβ
1−42 (10 μM), LPS (5 ng/mL), or Aβ
1−42 (10 μM) plus LPS (5 ng/mL) was added as
indicated, and 6 hours later, total RNA was isolated. CD14 (a), MyD88 (b), TLR4 (c), and TLR2 (d) mRNA levels were determined by real-time quantitative RT-PCR. Results are expressed as fold inductions in GAPDH
normalized mRNA values versus levels in LPS-treated cells. Values are
mean ± s.e.m of six samples derived from three independent
experiments, with each experiment performed in duplicate. *P < .05, **P < .01, and ***P < .001 versus Aβ
1−42 + LPS-treated cultures.