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. 2008 Jul 2;2008:349185. doi: 10.1155/2008/349185

Figure 4.

Figure 4

The effects of 15d-PGJ2 on microglial mRNA expression of CD14, MyD88, TLR4, and TLR2 induced by Aβ1−42 plus LPS. Cells were pretreated for 1 hour with 15d-PGJ2 (2.5 μM). Aβ 1−42 (10 μM), LPS (5 ng/mL), or Aβ 1−42 (10 μM) plus LPS (5 ng/mL) was added as indicated, and 6 hours later, total RNA was isolated. CD14 (a), MyD88 (b), TLR4 (c), and TLR2 (d) mRNA levels were determined by real-time quantitative RT-PCR. Results are expressed as fold inductions in GAPDH normalized mRNA values versus levels in LPS-treated cells. Values are mean ± s.e.m of six samples derived from three independent experiments, with each experiment performed in duplicate. *P < .05, **P < .01, and ***P < .001 versus Aβ 1−42 + LPS-treated cultures.