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. 1999 Dec 7;96(25):14288–14293. doi: 10.1073/pnas.96.25.14288

Figure 1.

Figure 1

Immunoblot using CP43 antibody to calculate contamination with CP43 in the CP47RC monomers and dimers. Loading was based on molar levels of the complexes. Lanes: 1, CP47RC monomer, 0.74 nmol; 2, CP47RC dimer, 0.67 nmol; 3, CP47RC dimer, 1.3 nmol; 4, CP43, 7.5 pmol; 5, CP43, 35 pmol; 6, CP43, 75 pmol. Using lanes 4–6 for a calibration curve, the CP43 contamination corresponds to 1.88 pmol in 0.67 nmol CP47RC dimers (lane 2) and 3.75 pmol in 1.3 nmol CP47RC dimers (lane 3), i.e., 0.3% contamination.