Figure 3.

Dependence of the t_lag for photoactivation (A) and maximal oxygen yield (B) of activated centers of CP47RC dimers (●) and apo-WOC-PSII membranes (□) on CaCl₂ concentration. For CP47RC dimers, measurements were conducted on samples in buffer containing 40 μM MnCl₂, protein equivalent to 10 μM RCs, and 0.8 mM ferricyanide. Light conditions are as shown in Fig. 2. The t_lag was estimated by using data from the first illumination cycle (see Fig. 2A), whereas the maximal oxygen evolution refers to the maximal yield at the end of a second illumination cycle. Data for apo-WOC-PSII membranes were taken from ref. 22.