Figure 5.

Maximal oxygen evolution rate of photoactivated CP47RC dimers (bars) and relative photoinhibition (♦) vs. dark time between the flashes. Reaction mixture contained 40 μM MnCl₂, 80 mM CaCl₂, protein equivalent to 10 μM RCs, and 0.8 mM ferricyanide. Photoinhibition was calculated as the relative loss of maximal oxygen evolution on the final flash of 405 ms of a third cycle of illumination with the yield on the last flash of the second cycle (both cycles measured under conditions identical with those of Fig. 2A, trace II).