Table 1.
ilvPy∷galK and ilvPC∷lacZ expression in wild-type and mutant strains
| Strain | Relevant genotype | Galactokinase-specific activity (ilvY) | β-Galactosidase-specific activity (ilvC) |
|---|---|---|---|
| IH5500 | Wild type | 0.65 ± 0.08 | 9.4 ± 1.4 |
| IH5510 | ΔilvY | 3.21 ± 0.29 | 27.4 ± 1.4 |
| HI5550 | ilvPCdown | 0.49 ± 0.08 | ND |
| IH5560 | ilvPCdown,ΔilvY | 2.45 ± 0.15 | ND |
| IH5580 | ilvPYdown | 0.05 ± 0.01 | 0.9 ± 0.1 |
| IH5590 | ilvPYdown,ΔilvY | 0.14 ± 0.01 | 4.0 ± 0.2 |
Galactokinase- and β-galactosidase-specific activities are expressed as the mean ± SD of six independent experiments. ND, not detectable.