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2a) BTL-1 and BTL-2 were previously reduced and carboxymethylated and later sequenced in an automatic gas-liquid sequencer Procise f (Applied Biosytem). 2b) The CD spectra were obtained using a J715 spectropolarimeter (Jarco Cop., Japan). BTL-2 was dissolved in water and the solution was adjusted to a final concentration of 10 mM of protein.
